Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) that was created by introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. It offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.
MMLV Reverse Transcriptase (MMLV RT), encoded by Moloney Murine Leukemia Virus is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. MLV RT will also extend primers hybridized to single-stranded DNA.
Low RNase H activity
efficient transcription of GC-rich targets
Catalog | YST-0003 | |||||||||
Shelf Life | 1 Year | |||||||||
Buffer | 50% gycerol | |||||||||
Shipping Temperature | -20°C | |||||||||
Quality Control | Certificate of Analysis (CoA) | |||||||||
Freeze/Thaw | No more than 3 freeze/thaw cycles. | |||||||||
Regulatory | For Research Use Only. Not intended for human or animal diagnostics or therapeutics. | |||||||||
Restrictions | This is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes. |