SYBR Green Real-Time One-Step RT-PCR Master Mix is a one-step, ready-to-use master mix for RNA quantification with SYBR Green detection. The optimized buffer formulation offers specificity, robustness, and reliability. The RT-PCR process converts and amplifies single-stranded RNA template yielding double-stranded DNA product. One-Step RT-PCR uses gene specific primers, designed to match RNA/cDNA targets, in a single-tube/plate, one-step reaction. This approach offers tremendous convenience when applied to analysis of single targets from multiple RNA samples. Also, it minimizes the possibility of introducing contaminants into reactions between the RT and PCR steps, since both steps are carried out sequentially without opening the reaction tubes/plates between the steps. Product Features: 1. Reproducible, consistent results while maintaining precision and efficiency. 2. Multiple platform compatibility with all real-time master mixes. 3. One-step, sequential reaction format for minimum hands on time. 4. Pre-mixed format reduces potential contamination. 5. Exceptional stability even after 10 freeze-thaw cycles.
Single-Tube Reaction
1-Step Master Mix
No reference dye
Catalog | YST-0017 | |||||||||
Shelf Life | 1 Year | |||||||||
Buffer | n/a | |||||||||
Quality Control | Certificate of Analysis (CoA) | |||||||||
Freeze/Thaw | No more than 10 freeze/thaw cycles. | |||||||||
Regulatory | For Research Use Only. Not intended for human or animal diagnostics or therapeutics. | |||||||||
Restrictions | This is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes. |