Quan-Plex™ NGS 10% Formalin-Compromised Reference Standard
Description:
The Quan-Plex™ FFPE NGS Reference Standard is a highly-characterized, quantitative multiplex, reference standard that allows researchers to accurately assess the quality of an oncology-based next-generation sequencing (NGS), digital PCR, and/or real-time PCR workflow with using CRISPR/cas9 genome-edited cancer cell lines that were fixed with 10% buffered formalin. This reference standard panel spans a broad range of actionable mutations at digital PCR and NGS-verifed mutation allelic frequencies (%MAF) ranging from 1% to 10%. In addition, the Quan-Plex™ NGS FFPE Reference Standard contains > 1,500 variants that are endogenous to the isogenic parental cancer cell lines (RKO and HCT116). This convenient and easy-to-use panel includes nine (9) actionable somatic mutations across six oncogenes (KIT, EGFR, NRAS, HRAS, KRAS and PIK3CA).
Price: $375.00
Datasheet
Variant Information:
| Gene | Nucleotide | Amino Acid | Cosmic | Frequency |
|---|---|---|---|---|
| EGFR | 2573T>G | L858R | COSM6224 | Homozygous |
| EGFR | c.2369C>T | p.T790M | COSM6240 | Homozygous |
| HRAS | c.182A>G | p.Q61R | COSM499 | Homozygous |
| KIT | c.2447A>T | p.D816V | COSM1314 | Homozygous |
| KRAS | 35G>A | G12D | COSM521 | Homozygous |
| NRAS | c.181C>A | p.Q61K | COSM580 | Homozygous |
| PIK3CA | c.1633G>A | p.E545K | COSM763 | Homozygous |
| PIK3CA | c.3140A>T | p.H1047L | COSM776 | Homozygous |
| EGFR | 2155G>A | G719S | COSM6252 | Homozygous |
General Information:
| Catalog | ARF-1001FC-1 | |||||||||
| Size | 1 vial | |||||||||
| Format | 10% Formalin-compromised gDNA | |||||||||
| Volume/Dimension | 40 µl | |||||||||
| Quantity | 1 µg | |||||||||
| Concentration | 25 ng/µl | |||||||||
| Cell(s) | RKO, HCT116 | |||||||||
| Shelf Life | 36 months | |||||||||
| Buffer | Tris-EDTA (10 mM Tris-HCl, 1 mM EDTA), pH 8.0 | |||||||||
| Shipping Temperature | Ambient | |||||||||
| Fixation Process | 10% buffered formalin solution (4% formaldehyde) for 24 hours at room temperature | |||||||||
| Storage Temperature | -20°C | |||||||||
| Quality Control | Genotype: Sanger sequencing of locus-specific PCR (cell line) Quality: DNA Quantification: Qubit 4.0 fluorometer Mutation Allelic Frequency: Digital PCR and NGS (Ion AmpliSeq Hotspot Cancer Panel V2 on Illumina) Manufacturing: ISO 9001:2015 and ISO 13485:2016 Certified |
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| Freeze/Thaw | No more than 3 cycles | |||||||||
| Regulatory | Research Use Only. Not Intended for human or animal diagnostic or therapeutic use. | |||||||||
| Restrictions | This is a limited use product. This product, any material that contains this product in whole or in part, any progeny, modification or derivative of this product, any cell or animal made or modified by using this product, and any progeny, modification or derivative of such cell or animal may not be transferred by the purchaser to any other person, entity or any of the above to perform services for the benefit of any other person or entity or for commercial purpose of any kind. This product may only be used by the purchaser for its internal research for use as a research tool for research purposes. | |||||||||
| Catalog: | ARF-1001FC-1 |
| Price: | $375.00 |
| Availability: | comming soon |
