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Custom Services

Onco-Ref™ Custom FFPE Cell Block Services

Our Onco-Ref™ Custom FFPE Cell Block Services gives you the flexibilty to receive a custom FFPE product with high quality and consistency with a fast turnaround time at a great price. Simply ship us your cells. If needed, we can culture, fix the cells using buffered formalin (10% for 24hrs at room temperature, typically), and then prepare a cell core and embed the core into a paraffin block. If requested (optional), we can prepare FFPE Cell slides or FPPE Cell scrolls from a freshly prepared FFPE Cell Block. We typically turnaround a FFPE Cell block in 7 working days from receipt of 1 x 108 cells.


How to obtain a custom FFPE Cell Block Quote:
  1. Download our custom FFPE Services Order Form
  2. Save the PDF with a different file name
  3. Open the PDF form (with the new file name)
  4. Click the "Submit" button located on the top of the order form.
Download Order Form
The Process
  1. Choose Cell Lines
  2. Onco-Ref™ Mutation Cell Line
  3. Human Cancer Cell Lines Offered (see list below)
    • Breast Cancer (SK-BR-3, MCF7, MDA-MB-231, MDA-MB-468, BT-474, T-47D
    • Lung Cancer (H2228, H23, A549, H460, HOP-62, H322)
    • Cervical Cancer (HeLa, Ca Ski)
    • Prostate Cancer (PC3, DU-145)
    • Colon Cancer (SW-620, HT-29, HCC-2998)
    • Melanoma (SK-MEL-2, SK-MEL-5, M14, M21, CHL-1)
    • Leukemia (K-562, Jurkat, Raji)
    • Ovarian Cancer (OVCAR-8, IGR-OV1)
    • Renal Cancer (SN-12C, TK-10)
    • Liver Cancer (HepG2, Huh-7)

Typical FFPE Cell Block Preparation Protocol:
  • Fixation: 10% formaldehyde/1xPBS for 24 hours at 24-26°C
  • Section thickness: 5 µm
  • Cell Core: 5.0 mm diameter
  • Cell density: >75%
  • Slide type: SuperFrostTM Plus

To learn more about our Onco-RefTM FFPE Reference Standards and other diagnostic products, WATCH for our webinar titled "Onco-Ref™ Reference Standards: Application of CRISPR/Cas9 to the Generation of Isogenic Cell Lines and Reference Materials".

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