Custom Services

Onco-Ref™ Custom FFPE Cell Block Services

Our Onco-Ref™ Custom FFPE Cell Block Services gives you the flexibilty to receive a custom FFPE product with high quality and consistency with a fast turnaround time at a great price. Simply ship us your cells. If needed, we can culture, fix the cells using buffered formalin (10% for 24hrs at room temperature, typically), and then prepare a cell core and embed the core into a paraffin block. If requested (optional), we can prepare FFPE Cell slides or FPPE Cell scrolls from a freshly prepared FFPE Cell Block. We typically turnaround a FFPE Cell block in 7 working days from receipt of 1 x 108 cells.

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How to obtain a custom FFPE Cell Block Quote:

1. Download our custom FFPE Services Order Form
2. Save the PDF with a different file name
3. Open the PDF form (with the new file name)
4. Click the "Submit" button located on the top of the order form.

Download Order Form

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The Process

1. Choose Cell Lines
2. Onco-Ref™ Mutation Cell Line
3. Human Cancer Cell Lines Offered (see list below)
• Breast Cancer (SK-BR-3, MCF7, MDA-MB-231, MDA-MB-468, BT-474, T-47D
• Lung Cancer (H2228, H23, A549, H460, HOP-62, H322)
• Cervical Cancer (HeLa, Ca Ski)
• Prostate Cancer (PC3, DU-145)
• Colon Cancer (SW-620, HT-29, HCC-2998)
• Melanoma (SK-MEL-2, SK-MEL-5, M14, M21, CHL-1)
• Leukemia (K-562, Jurkat, Raji)
• Ovarian Cancer (OVCAR-8, IGR-OV1)
• Renal Cancer (SN-12C, TK-10)
• Liver Cancer (HepG2, Huh-7)

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Typical FFPE Cell Block Preparation Protocol:

• Fixation: 10% formaldehyde/1xPBS for 24 hours at 24-26°C
• Section thickness: 5 µm
• Cell Core: 5.0 mm diameter
• Cell density: >60%
• Slide type: SuperFrostTM Plus

To learn more about our Onco-RefTM FFPE Reference Standards and other diagnostic products, WATCH for our webinar titled "Onco-Ref™ Reference Standards: Application of CRISPR/Cas9 to the Generation of Isogenic Cell Lines and Reference Materials".